6-ethyl-7-hydroxy-4-methylchromen-2-one

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

2-(4-Hydroxyphenylazo)benzoicacid is a spectrophotometric probe that shows changes in absorption spectra upon binding to proteins and binds to bovine serum proteins.

9-Amino-6-chloro-2-methoxyacridine serves as a pH-sensitive fluorescent probe commonly utilized to assess vacuolar pH variations upon substrate translocation across the tonoplast via a putative H+/solute antiport system [1].

Pyridine-2-carboxaldehyde 4-nitrophenylhydrazone (PCNPH) serves as a chromogenic substrate for peroxidase enzymes, reacting to form a purple indamine dye in the presence of peroxidases and peroxides [1].

4-Hydrazino-7-nitrobenzofurazan hydrazine adduct serves as a fluorescent reagent for protein labeling.

4-(2-Aminoethylamino)-7-(N,N-dimethylsulfamoyl)benzofurazan serves as a precolumn fluorescence derivatization reagent.

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient. 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.

N-[4-(2-Benzimidazolyl)phenyl]maleimide serves as a potent fluorescent probe featuring an excitation maximum at 315 nm and emission maximum at 360 nm, suitable for the fluorometry of thiol compounds [1].

4-(Benzyloxy)-2-nitrophenol serves as a synthetic precursor for constructing fluorescent ion indicators [1].

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside (4-MU-α-GlcNS) is a fluorogenic substrate of heparin sulphamidase. Heparin sulphamidase cleaves 4-MU-α-GlcNS to yield 4-MU-α-GlcNH2, which is then cleaved by α-glucosaminidase to release the fluorescent product 4-MU, which displays an emission maxima of 445-454 nm. The excitation maxima for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. 4-MU-α-GlcNS has been used to quantify heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders.

7-Acetoxy-4-bromomethylcoumarin serves as a fluorescent label for fatty acids.

7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation/emission maxima of 380/515 nm, respectively.1 1.Imai, K., Toyo’oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009)

7-Ethoxy-5-(trifluoromethyl)coumarin is a fluorogenic substrate for cytochrome P450s (CYPs).1Upon O-deethylation by CYPs, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP activity. HFC displays excitation/emission maxima of 410/510 nm, respectively. 1.Buters, J.T.M., Schiller, C.D., and Chou, R.C.A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarinBiochem. Pharmacol.46(9)1577-1584(1993)

AMCA-X, N-succinimidyl ester is an amine-reactive, UV-excitable, blue fluroscent dye.

MESG, a chromophoric substrate, can be used to quantitate inorganic phosphate.

2,5-Dioxopyrrolidin-1-yl 2-(7-hydroxy-4-methyl-2-oxo-2H-chromen-3-yl)acetate is a useful organic compound for research related to life sciences. The catalog number is T67683 and the CAS number is 96735-88-5.

7-Hydroxy-4-methylcoumarin-3-acetic acid is a blue fluorophore that has pH-dependent and environment-sensitive fluorescence. This coumarin is increasingly used to label peptides, nucleotides and carbohydrates.

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

Sodium 2,2',2'',2'''-((((1,1-dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-isopropyl-2-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetate is a useful organic compound for research related to life sciences. The catalog number is T65315 and the CAS number is 1945-77-3.

7-(Diethylamino)-4-methyl-2H-chromen-2-one is a useful organic compound for research related to life sciences. The catalog number is T66262 and the CAS number is 91-44-1.

7-Dimethylaminocoumarin-4-acetic acid is a useful organic compound for research related to life sciences. The catalog number is T64950 and the CAS number is 80883-54-1.

L-Leu-AMC is a fluorogenic substrate for leucine aminopeptidase.1Upon enzymatic cleavage by leucine aminopeptidase, AMC is released and its fluorescence can be used to quantify leucine aminopeptidase activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. 1.Izquierdo, M., Lin, D., O’Neill, S., et al.Development of a high-throughput screening assay to identify inhibitors of the major M17-leucyl aminopeptidase from Trypanosoma cruzi using rapidfire mass spectrometrySLAS Discov.25(9)1064-1071(2020)

2-(7-Hydroxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T65277 and the CAS number is 6950-82-9.

2-(7-Methoxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T64951 and the CAS number is 62935-72-2.

Sodium 2,6-dibromo-4-(3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-2,2-dioxido-3H-benzo[d][1,2]oxathiol-3-yl)-3-methylphenolate is a useful organic compound for research related to life sciences. The catalog number is T64788 and the CAS number is 62625-32-5.

Flutax-2 (5/6-mixture), an active fluorescent derivative of paclitaxel, binds to polymerized α,β tubulin dimers, with an excitation/emission wavelength of 496/524 nm. Derived from paclitaxel, a diterpenoid secondary metabolite from Taxus species utilized in various cancer research [1], it requires storage protection from light.

7-Hydroxy-4-methyl-2(1H)-quinolone (compound 2b), a fluorescent hydroxylated derivative, serves as a probe for detecting hydroxyl radicals associated with DNA damage [1]. For preservation, it should be protected from light.

2-([1,1-Biphenyl]-4-yl)-5-phenyl-1,3,4-oxadiazole is a useful organic compound for research related to life sciences. The catalog number is T66233 and the CAS number is 852-38-0.

Sodium 4-(3-(4-hydroxy-5-isopropyl-2-methylphenyl)-1,1-dioxido-3H-benzo[c][1,2]oxathiol-3-yl)-2-isopropyl-5-methylphenolate is a useful organic compound for research related to life sciences. The catalog number is T66669 and the CAS number is 62625-21-2.