7fluoro2,1,3benzoxadiazole4sulfonate (ammonium salt)

7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation/emission maxima of 380/515 nm, respectively.1 1.Imai, K., Toyo’oka, T., and Watanabe, Y.A novel fluorogenic reagent for thiols: Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonateAnal. Biochem.128(2)471-473(1983) 2.Ichinose, S., Nakamura, M., Maeda, M., et al.A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasmaBiomed. Chromatogr.23(9)935-939(2009)

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

1-Methyl-7-nitroisatoic anhydride (1M7) is a reagent. It detects local nucleotide flexibility, for probing 2'-hydroxyl reactivity.

4-Di-1-ASP, a styryl dye, selectively stains glioma cells within live brain tissue, facilitating microscopic analysis of cell structure, viability, and processes including proliferation, endocytosis, cytokinesis, and phagocytosis. It also allows for the observation of mitochondrial structures in living cells. The dye exhibits green fluorescence when visualized under a microscope (λ ex λ em = 475 606 nm) [1] [2].

Mag-Indo-1 tetrapotassium salt is a cell-impermeable fluorescent probe designed for detecting Mg2+ [1].

4-(2-Aminoethylamino)-7-(N,N-dimethylsulfamoyl)benzofurazan functions as a precolumn fluorescence derivatization reagent.

4-Hydrazino-7-nitrobenzofurazan hydrazine adduct serves as a fluorescent reagent for protein labeling.

Cal Green 1 is a cell-impermeant fluorescent calcium indicator that is characterized by high quantum yield and low phototoxicity. Its peak excitation and emission wavelengths (506 and 531 nm, respectively) are comparable to standard fluorescein dyes, making Cal Green 1 appropriate for fluorescent microscopy. Cal Green 1 is ~5-fold brighter than fluo-3 at saturating calcium levels.

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient. 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.

7-Ethoxy-5-(trifluoromethyl)coumarin is a fluorogenic substrate for cytochrome P450s (CYPs).1Upon O-deethylation by CYPs, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP activity. HFC displays excitation/emission maxima of 410/510 nm, respectively. 1.Buters, J.T.M., Schiller, C.D., and Chou, R.C.A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarinBiochem. Pharmacol.46(9)1577-1584(1993)

Dipotassium nitrocatechol sulfate (4-Nitrocatechol sulfate dipotassium salt) acts as a chromogenic substrate for sulphatase [1].

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

N-(7-Nitrobenzofurazan-4-yl)phallacidin is a fluorochrome and serves as a visualizing probe for actin [1].

Fluo-3 (ammonium salt) is a fluorescent calcium indicator commonly used in flow cytometry and cell-based experiments to detect changes in intracellular calcium levels. [1] Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.

7-Hydroxy-4-methylcoumarin-3-acetic acid, SE is a useful organic compound for research related to life sciences. The catalog number is T67683 and the CAS number is 96735-88-5.

7-Hydroxy-4-methylcoumarin-3-acetic acid is a blue fluorophore with pH-dependent and environment-sensitive fluorescence, increasingly used to label peptides, nucleotides, and carbohydrates.

7-(Diethylamino)-4-methyl-2H-chromen-2-one is a useful organic compound for research related to life sciences. The catalog number is T66262 and the CAS number is 91-44-1.

AMCA-X, N-succinimidyl ester is an amine-reactive, UV-excitable, blue fluroscent dye.

4-(1-Methylhydrazino)-7-nitrobenzofurazan serves as a versatile dye with applications extending from basic scientific research to clinical diagnostics. Dyes, crucial for various biological experiments, enable scientists to examine cell structures, track biomolecules, assess cell functionality, identify different cell types, detect biomolecules, explore tissue pathology, and observe microorganisms. Beyond scientific research, dyes are prevalent in traditional sectors like textile dyeing and emerging areas, including functional textile processing, food pigments, and dye-sensitized solar cells.

7-Diethylamino-3-N-(4-maleimidopropyl)carbamoylcoumarin is a versatile dye utilized across various fields. This compound is primarily important in biological experiments for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its applications extend beyond basic scientific research into clinical diagnostics. Additionally, it is employed in traditional industries like textile dyeing and in innovative areas including functional textile processing, food pigments, and dye-sensitized solar cells.

5-Bromo-4-chloro-3-indolyl-b-D-glucuronide sodium salt is a useful organic compound for research related to life sciences. The catalog number is T64896 and the CAS number is 129541-41-9.

7-Dimethylaminocoumarin-4-acetic acid, catalog number T64950, CAS number 80883-54-1, is a valuable organic compound for life sciences research.

L-Leu-AMC is a fluorogenic substrate for leucine aminopeptidase.1Upon enzymatic cleavage by leucine aminopeptidase, AMC is released and its fluorescence can be used to quantify leucine aminopeptidase activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. 1.Izquierdo, M., Lin, D., O’Neill, S., et al.Development of a high-throughput screening assay to identify inhibitors of the major M17-leucyl aminopeptidase from Trypanosoma cruzi using rapidfire mass spectrometrySLAS Discov.25(9)1064-1071(2020)

4,4'-((4-Iminocyclohexa-2,5-dien-1-ylidene)methylene)dianiline hydrochloride is a useful organic compound for research related to life sciences. The catalog number is T64740 and the CAS number is 569-61-9.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

2-(7-Hydroxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T65277 and the CAS number is 6950-82-9.

2-(7-Methoxy-2-oxo-2H-chromen-4-yl)acetic acid is a useful organic compound for research related to life sciences. The catalog number is T64951 and the CAS number is 62935-72-2.

Indo-1 (potassium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (potassium salt) binds calcium. Indo-1 (potassium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.

C.I. Direct Red 1, disodium salt is a dye.

Sodium diphenylamine-4-sulfonate is an oxidation-reduction titration indicator, displaying a colorless reduced form and a red-violet oxidized form [1].

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside (4-MU-α-GlcNS) is a fluorogenic substrate of heparin sulphamidase. Heparin sulphamidase cleaves 4-MU-α-GlcNS to yield 4-MU-α-GlcNH2, which is then cleaved by α-glucosaminidase to release the fluorescent product 4-MU, which displays an emission maxima of 445-454 nm. The excitation maxima for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. 4-MU-α-GlcNS has been used to quantify heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders.

4-Nitro-7-piperazinobenzofurazan serves as a multifunctional dye, crucial in both scientific and industrial applications. As invaluable tools in biological experiments, dyes enable researchers to study cell structures, track biomolecules, assess cell functions, differentiate between cell types, detect biomolecules, analyze tissue pathology, and monitor microorganisms. Their utility extends beyond basic research into clinical diagnostics and various traditional uses including textile dyeing. Furthermore, dyes play significant roles in innovative sectors like functional textile processing, food coloring, and dye-sensitized solar cells.

4-Amino-3-hydroxynaphthalene-1-sulfonic acid serves as a fluorescent dye and is employed in the formation of polypyrrole (PPy) conducting films with pyrrole for use in electrochemie [1] [2].

N-(4-Anilino-1-naphthyl)maleimide serves as a fluorescent probe for detecting thiol groups in proteins, reacting with these groups to instantly form a stable fluorescent compound characterized by λ ex = 355 nm and λ em = 448 nm [1].

12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid functions as an amine-reactive fluorescent dye [1].

7-Acetoxy-4-bromomethylcoumarin serves as a fluorescent label for fatty acids.

Indo-1 (sodium salt) is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when Indo-1 (sodium salt) binds calcium. Indo-1 (sodium salt) is prone to photobleaching, which limits its usefulness in methods involving microscopy.

N-(4-(Bis(4-(diethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)-N-ethylethanaminium chloride is a useful organic compound for research related to life sciences. The catalog number is T65162 and the CAS number is 2390-59-2.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentasodium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

4-(7-Diethylaminocoumarin-3-yl)benzoyl cyanide (DACB-CN) serves as a multifunctional dye crucial for a wide array of applications. This chemical compound is utilized extensively in biological experiments to monitor and analyze various cellular components and activities. It aids in observing cell structures, tracking biomolecules, assessing cell functions, differentiating cell types, detecting biomolecules, examining tissue pathology, and studying microorganisms. Beyond its scientific research applications, DACB-CN is also employed in traditional industries like textile dyeing and in innovative sectors, including functional textile processing, food pigments, and dye-sensitized solar cells.

7-Amino-4-methylcoumarin hydrogensulfate (Coumarin 120 hydrogensulfate; AMC hydrogensulfate) serves as a versatile dye extensively utilized across various domains. This compound plays a crucial role in biological experiments, aiding in the observation and analysis of cell structures, tracking of biomolecules, evaluation of cell functions, differentiation of cell types, detection of biomolecules, examination of tissue pathology, and monitoring of microorganisms. Its applications extend beyond basic scientific research, encompassing both traditional uses in textile dyeing and contemporary usages in functional textile processing, food pigments, and dye-sensitized solar cells.

4-Fluoro-2,1,3-benzoxadiazol, also known as 4-Fluorobenzofurazan, is a derivative of benzofurazan characterized by its fluorescent properties [1].

7-Hydroxy-4-methyl-2(1H)-quinolone (compound 2b), a fluorescent hydroxylated derivative, serves as a probe for detecting hydroxyl radicals associated with DNA damage [1]. It should be protected from light for preservation.