5(and6)carboxy snarf1, acetoxymethyl ester, acetate

The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent

1-Naphthyl acetate is usually used in a rapid staining method for identification of macrophages. 1-Naphthyl acetate is a potent chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. It has the potential to detect organophosphorus pesticide poisoning.

6-CFDA N-succinimidyl ester (Carboxyfluorescein diacetate succinimidyl) is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

5(6)-Aminofluorescein (5(6)-AFM) is a high-purity, high-quality chemical utilized as a molecular probe and a significant fluorescent agent.

Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 489 nm excitation wavelength. They are photostable alternative reag

5-FAM-β-Amyloid (1-28) is a peptide labeled with a fluorescent probe, representing the initial 28 amino acids of the β-Amyloid protein.

1,N6-Ethenoadenosine 5'-monophosphate sodium (1,N6-Etheno-AMP sodium), a fluorescent analog of adenosine 5'-monophosphate (AMP), serves as an effective probe for AMP-related systems due to its detectability at low concentrations. It is characterized by an excitation wavelength range of 250-300 nm and emits at 415 nm [1] [2].

NHS-5(6)Carboxyrhodamine is a fluorescent dye used for precise protein labeling through fluorescence, ensuring accurate dye-to-protein ratios even under native conditions.

(5)6-Carboxytetramethylrhodamine, a bright orange-fluorescent dye with absorption emission maxima of ~555 580 nm [1], contains a carboxylic acid that reacts with primary amines via carbodiimide activation.

5(6)-Carboxynaphthofluorescein, a pH-dependent fluorophore, exhibits high sensitivity in alkaline pH settings, making it suitable for the development of fiber-optic pH sensors. Additionally, it serves as a fluorescent pH indicator with excitation emission wavelengths of 593 668 nm [1].

5(6)-Carboxy-2',7'-dichlorofluorescein serves as an excellent substrate for the MRP2 vesicular transport assay, offering superior detection and transport characteristics [1].

5-Nitro BAPTA tetramethyl ester, a calcium chelator, is involved in the synthesis of two-photon probes and is used for real-time imaging of intracellular calcium ions and monitoring calcium waves at depths of 100-300 μm in liver tissues for 1100-4000 s. Additionally, in conjunction with fluorescent compound 2-Me-substituted TM, it can form a red fluorescent probe (CaTM-2 AM) [1] [2].

6-Carboxy-JF5252 is a fluorophore that can be used as probes, dyes, and tags for super-resolution imaging research [1].

ROX dyes have a strong red fluorescence.Their excitation and emission wavelengths are longer than those of traditional rhodamine dyes.They are used as labels for peptides, proteins, and other biological ligands.5-ROX, 6-ROx, and their mixture of 5(6) -RoX

6-CR110, SE [6-Carboxyrhodamine 110, succinimidyl ester]*Single isomer* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

Sodium 2,2',2'',2'''-((((1,1-dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-isopropyl-2-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetate is a useful organic compound for research related to life sciences. The catalog number is T65315 and the CAS number is 1945-77-3.

5(6)-ROX, a nucleic acid fluorescent label, serves as a reference dye in real-time polymerase chain reaction (PCR) applications.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

5-Carboxyrhodamine 6G succinimidyl ester (R6G-SE) functions as an amine-reactive fluorescent dye [1].

5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

2-(Naphthalen-1-yl)-5-phenyloxazole (α-NPO) is an organic fluorescent dye featuring a nitrogen atom, potentially interacting with nitrogen introduced during the amination step [1].

HKSOX-1m hydrobromide (5/6-mixture), a mitochondria-targeted O2•− fluorescent probe (Ex/Em=509/534nm; green), demonstrates exceptional selectivity and sensitivity for O2•− across a wide pH spectrum, robustly distinguishing it from strong oxidants and prevalent cellular reductants [1].

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

5(6)-Carboxy-eosin, a multifunctional dye, serves as a crucial tool across various scientific, clinical, and industrial applications. In biological experiments, this dye enables researchers to observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish between cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Its utility extends beyond basic research to encompass a broad spectrum of diagnostics. Additionally, 5(6)-Carboxy-eosin finds extensive use in traditional sectors like textile dyeing and in innovative areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

Mca-(endo-1α-Dap(Dnp))-TNF-α(-5 to +6) amide (human) TFA is a peptide that functions as a fluorescence resonance energy transfer (FRET)-based substrate, with its activity determined by the change in fluorescence intensity following cleavage [1].

HKSOX-1, a fluorescent probe designed for the imaging and detection of endogenous superoxide in live cells and in vivo, demonstrates outstanding selectivity and sensitivity towards the superoxide anion radical [1]. For storage, it is important to protect HKSOX-1 from light.

5(6)-ROX, SE is an amine-reactive fluorescent probe that is widely used for oligonucleotide labeling and automated DNA sequencing applications.

5(6)-CR110, SE [5-(and 6)-Carboxyrhodamine 110, succinimidyl ester] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm.

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester (cDCFDASE) serves as a fluorescent probe for assessing the intracellular pH (pH in) in living cells under various external pH conditions (pH ex) [1]. This compound is utilized to measure pH dynamics within cells, facilitating critical insights into cellular function and behavior.

ROX NHS ester, 6-isomer, is a highly fluorescent and photostable rhodamine dye used for labeling amino groups in peptides, proteins, and amino-oligonucleotides. ROX-labeled oligonucleotide probes are commonly used in qPCR, and qPCR instruments have a ROX channel. [Pure single isomer.]

5(6)-Carboxyfluorescein diisobutyrate, a protected form of 5(6)-carboxyfluorescein, serves as a fluorescence pH indicator [1].

2'-Deoxy-1,N6-ethenoadenosine-5'-O-triphosphate (ε-dATP), a fluorescent derivative of 2’-deoxyadenosine-5’-O-triphosphate (dATP), promotes sister chromatid exchange in stimulated primary mouse spleen T- and B lymphocytes with LPS or concanavalin A at concentrations of 50 to 150 µM. Utilized in ambiguous base pairing investigations within vinyl chloride-induced mutagenesis research, ε-dATP, along with its related compound ε-ATP—which exhibits an emission peak at 415 nm after being excited at 300 nm—contributes to understanding genetic alterations mechanisms.

5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm.

5(6)-TRITC [Tetramethylrhodamine-5-(and-6) -Isothiocyanate] *Mixed isomers* is used to label antibodies with strong fluorescent dyes

HKSOX-1r (5 6-mixture) is a fluorescent probe specifically designed for the imaging and detection of endogenous superoxide in live cells and in vivo applications, demonstrating superior selectivity and sensitivity to the superoxide anion radical [1]. For storage, it must be protected from light.

HKSOX-1m (5 6-mixture) is a mitochondria-targeting O2 fluorescent probe with excitation emission wavelengths of 509 534 nm (green), offering exceptional selectivity and sensitivity for O2 across a wide pH range and in the presence of strong oxidants and prevalent reductants within cells.

5-(6)-Carboxy RhodFluor is a cell-impermeant pH indicator.

5-TAMRA-Amyloid β-Protein (1-40) is a TAMRA-labeled variant of Amyloid β-Protein (1-40) with an absorption emission spectrum of 544 572 nm [1].

Flutax-2 (5/6-mixture), an active fluorescent derivative of paclitaxel, binds to polymerized α,β tubulin dimers, with an excitation/emission wavelength of 496/524 nm. Derived from paclitaxel, a diterpenoid secondary metabolite from Taxus species utilized in various cancer research [1], it requires storage protection from light.

5(6)-Carboxyeosin-diacetate serves as a versatile dye with a broad spectrum of applications in both traditional and emerging fields. This compound is vital in biological experiments for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its utility extends beyond basic scientific research to encompass clinical diagnostics. Additionally, dyes like 5(6)-Carboxyeosin-diacetate are essential in industries such as textile dyeing, functional textile processing, food coloring, and the development of dye-sensitized solar cells.

5(6)-SFX, SE (Compound SFX-SE) is a fluorescent dye used for labeling primary amines and nascent proteins [1] [2].

5(6)-Carboxynaphthofluorescein diacetate serves as a fluorescent substrate in esterase assays, where it is cleaved by intracellular esterases, resulting in red fluorescence for enzyme activity measurement (Ex = 590 nm; Em = 645 nm) [1].