5(6) fitc

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

5(6)-Aminofluorescein (5(6)-AFM) is a high-purity, high-quality chemical utilized as a molecular probe and a significant fluorescent agent.

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-CR110, SE [5-(and 6)-Carboxyrhodamine 110, succinimidyl ester] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm.

5(6)-Carboxyeosin-diacetate serves as a versatile dye with a broad spectrum of applications in both traditional and emerging fields. This compound is vital in biological experiments for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its utility extends beyond basic scientific research to encompass clinical diagnostics. Additionally, dyes like 5(6)-Carboxyeosin-diacetate are essential in industries such as textile dyeing, functional textile processing, food coloring, and the development of dye-sensitized solar cells.

5(6)-Carboxynaphthofluorescein diacetate serves as a fluorescent substrate in esterase assays, where it is cleaved by intracellular esterases, resulting in red fluorescence for enzyme activity measurement (Ex = 590 nm; Em = 645 nm) [1].

5(6)-SFX, SE (Compound SFX-SE) is a fluorescent dye used for labeling primary amines and nascent proteins [1] [2].

5(6)-TRITC [Tetramethylrhodamine-5-(and-6) -Isothiocyanate] *Mixed isomers* is used to label antibodies with strong fluorescent dyes

HKSOX-1r (5 6-mixture) is a fluorescent probe specifically designed for the imaging and detection of endogenous superoxide in live cells and in vivo applications, demonstrating superior selectivity and sensitivity to the superoxide anion radical [1]. For storage, it must be protected from light.

Flutax-2 (5/6-mixture), an active fluorescent derivative of paclitaxel, binds to polymerized α,β tubulin dimers, with an excitation/emission wavelength of 496/524 nm. Derived from paclitaxel, a diterpenoid secondary metabolite from Taxus species utilized in various cancer research [1], it requires storage protection from light.

HKSOX-1m (5 6-mixture) is a mitochondria-targeting O2 fluorescent probe with excitation emission wavelengths of 509 534 nm (green), offering exceptional selectivity and sensitivity for O2 across a wide pH range and in the presence of strong oxidants and prevalent reductants within cells.

5-(6)-Carboxy RhodFluor is a cell-impermeant pH indicator.

The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent

5(6)-Carboxy-eosin, a multifunctional dye, serves as a crucial tool across various scientific, clinical, and industrial applications. In biological experiments, this dye enables researchers to observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish between cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Its utility extends beyond basic research to encompass a broad spectrum of diagnostics. Additionally, 5(6)-Carboxy-eosin finds extensive use in traditional sectors like textile dyeing and in innovative areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

6-Fluorescein Isothiocyanate (6-FITC) is a fluorescein derivative widely employed in applications such as flow cytometry.

HKSOX-1m hydrobromide (5/6-mixture), a mitochondria-targeted O2•− fluorescent probe (Ex/Em=509/534nm; green), demonstrates exceptional selectivity and sensitivity for O2•− across a wide pH spectrum, robustly distinguishing it from strong oxidants and prevalent cellular reductants [1].

ROX dyes have a strong red fluorescence.Their excitation and emission wavelengths are longer than those of traditional rhodamine dyes.They are used as labels for peptides, proteins, and other biological ligands.5-ROX, 6-ROx, and their mixture of 5(6) -RoX

Sodium 2,2',2'',2'''-((((1,1-dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-isopropyl-2-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetate is a useful organic compound for research related to life sciences. The catalog number is T65315 and the CAS number is 1945-77-3.

5(6)-ROX, a nucleic acid fluorescent label, serves as a reference dye in real-time polymerase chain reaction (PCR) applications.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

5(6)-ROX, SE is an amine-reactive fluorescent probe that is widely used for oligonucleotide labeling and automated DNA sequencing applications.

Mca-(endo-1α-Dap(Dnp))-TNF-α(-5 to +6) amide (human) TFA is a peptide that functions as a fluorescence resonance energy transfer (FRET)-based substrate, with its activity determined by the change in fluorescence intensity following cleavage [1].

5(6)-Carboxyfluorescein diisobutyrate, a protected form of 5(6)-carboxyfluorescein, serves as a fluorescence pH indicator [1].

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester (cDCFDASE) serves as a fluorescent probe for assessing the intracellular pH (pH in) in living cells under various external pH conditions (pH ex) [1]. This compound is utilized to measure pH dynamics within cells, facilitating critical insights into cellular function and behavior.

HKSOX-1, a fluorescent probe designed for the imaging and detection of endogenous superoxide in live cells and in vivo, demonstrates outstanding selectivity and sensitivity towards the superoxide anion radical [1]. For storage, it is important to protect HKSOX-1 from light.

5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is bright, orange-fluorescent dye produces conjugates with absorption emission maxima of ~555 580 nm.

Fmoc-Lys(5-FITC)-OH serves as a fluorescent marker for polypeptides and proteins, utilizing FITC—a pH and Cu^2+-sensitive dye—as its fluorescence probe for amine labeling [1] [2] [3].

NHS-5(6)Carboxyrhodamine is a fluorescent dye used for precise protein labeling through fluorescence, ensuring accurate dye-to-protein ratios even under native conditions.

(5)6-Carboxytetramethylrhodamine, a bright orange-fluorescent dye with absorption emission maxima of ~555 580 nm [1], contains a carboxylic acid that reacts with primary amines via carbodiimide activation.

5(6)-Carboxy-2',7'-dichlorofluorescein serves as an excellent substrate for the MRP2 vesicular transport assay, offering superior detection and transport characteristics [1].

5(6)-Carboxynaphthofluorescein, a pH-dependent fluorophore, exhibits high sensitivity in alkaline pH settings, making it suitable for the development of fiber-optic pH sensors. Additionally, it serves as a fluorescent pH indicator with excitation emission wavelengths of 593 668 nm [1].