5(6)tamra [5(and6)carboxytetramethylrhodamine] *mixed isomers*

5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm.

CY7-SE Triethylamine is a fluorescence labeling agent (Ex=700-770 nm，Em=790 nm) used for labeling proteins, peptides, antibodies, and oligonucleotides.

5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside is a chromogenic compound used to detect cellobiohydrolases.

6-Ethyl-7-hydroxy-4-methylchromen-2-one is a fluorescent dye.

5-ROX, SE ([5-Carboxy-X-rhodamine, succinimidyl ester] *Single isomer*) is a labeling reagen used in the preparation of charge-modified dye-labeled Dideoxynucleotide Triphosphates (ddNTP) to direct-load DNA sequencing.

6-CFDA N-succinimidyl ester (Carboxyfluorescein diacetate succinimidyl) is capable of spontaneously and irreversibly binding to free amines. Utilized in flow cytometry experiments for tracking cell division in both mammalian cells and bacteria. Used in combination with several cell permeabilizers as an effective, nondestructive fluorescencent stain to quickly detect bacterial cells.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

6-FAM SE (6-Carboxyfluorescein N-succinimidyl ester) is another isomer of carboxyfluorescein. 6-FAM SE is mainly used for labeling nucleotides and sequencing nucleic acids.

5-ROX (5-Carboxy-X-rhodamine), a rhodamine dye, exhibits strong fluorescence in aqueous buffer with an excitation wavelength (λexit) of 580 nm (ε = 3.6×10^4 (M·cm)) and an emission wavelength (λemit) of 604 nm (ϕ = 0.94).

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

5-FAM SE is a single isomer widely employed in the preparation of various small fluorescent molecules. It is a highly popular green fluorescent reagent used for labeling peptides, proteins, and nucleotides [5-FAM SE].

5-TAMRA (5-Carboxytetramethylrhodamine) (5-Carboxytetramethylrhodamine) yields bright, pH-insensitive orange-red fluorescence (approximate excitation emission maxima ~546 579) with good photostability.

6-ROX (6-Carboxy-X-rhodamine) is a fluorescent oligonucleotide marker and acts as an acceptor molecule coupled to 5-FAM as the donor in FRET imaging with excitation of 568nm and emission of 568nm.

5-Aminofluorescein (5-AF) (5-AF) is a covalently-bound fluorescence marker conjugated with human serum albumin. It exhibits an excitation wavelength of 490 nm and an emission wavelength of 515 nm.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

6-TAMRA (6-Carboxytetramethylrhodamine) serves as a popular fluorophore for the creation of bioconjugates, particularly in the synthesis of fluorescent antibodies and avidin derivatives employed in immunochemistry.

6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.

5(6)-Aminofluorescein (5(6)-AFM) is a high-purity, high-quality chemical utilized as a molecular probe and a significant fluorescent agent.

9-Amino-6-chloro-2-methoxyacridine is a pH-sensitive fluorescent probe commonly used to assess vacuolar pH variations during substrate translocation across the tonoplast through a putative H+ solute antiport system [1].

Fmoc-Lys(5-FITC)-OH serves as a fluorescent marker for polypeptides and proteins, utilizing FITC—a pH and Cu^2+-sensitive dye—as its fluorescence probe for amine labeling [1] [2] [3].

5-FAM maleimide, a derivative of 5-FAM, can be utilized for protein labeling [1].

5-CFDA-AM is a cell-permeable esterase substrate used as an active probe to evaluate enzyme activity and cell membrane integrity. Electroneutral and able to enter cells at lower concentrations than CFDA, it undergoes intracellular hydrolysis by esterases to produce carboxyfluorescein, which possesses an extra negative charge, enhancing cellular retention [1].

1,N6-Ethenoadenosine 5'-monophosphate sodium (1,N6-Etheno-AMP sodium), a fluorescent analog of adenosine 5'-monophosphate (AMP), serves as an effective probe for AMP-related systems due to its detectability at low concentrations. It is characterized by an excitation wavelength range of 250-300 nm and emits at 415 nm [1] [2].

NHS-5(6)Carboxyrhodamine is a fluorescent dye used for precise protein labeling through fluorescence, ensuring accurate dye-to-protein ratios even under native conditions.

5-Nitro BAPTA, a calcium chelator, combined with 2-Me-substituted TM (as a fluorescent moiety), forms a red fluorescent probe (CaTM-2 AM) for imaging cytoplasmic Ca 2+ in cultured living cells. It serves as a building block for synthesizing Ca 2+ specific chelators, Ca 2+ buffers, and fluorescent Ca 2+ indicators [1] [2].

5-Nitro BAPTA tetramethyl ester, a calcium chelator, is involved in the synthesis of two-photon probes and is used for real-time imaging of intracellular calcium ions and monitoring calcium waves at depths of 100-300 μm in liver tissues for 1100-4000 s. Additionally, in conjunction with fluorescent compound 2-Me-substituted TM, it can form a red fluorescent probe (CaTM-2 AM) [1] [2].

6-(4-Acetamido-1,8-naphthalamido)hexanoic acid is a versatile dye utilized across various domains. Employed extensively in biological experiments, this dye serves to analyze and monitor cell structures, differentiate cell types, and assess cellular functions. It aids in the detection of biomolecules, facilitates tissue pathology studies, and tracks microorganisms. Beyond basic research, its applications extend to clinical diagnostics and other fields including textile dyeing, functional textile processing, food coloring, and dye-sensitized solar cells.

2-N-Ethyl-p-(6-methoxybenzothiazol-2-yl)azoanilinoethanol serves as a versatile dye utilized across various domains. Dyes play a crucial role in biological experiments, enabling researchers to investigate and discern cell structures, monitor biomolecules, assess cellular functions, differentiate cell types, examine tissue pathology, and track microorganisms. Their utility extends beyond fundamental scientific research to include a broad spectrum of clinical diagnostics. Additionally, dyes are integral to conventional applications like textile dyeing and in newer areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

(5)6-Carboxytetramethylrhodamine, a bright orange-fluorescent dye with absorption emission maxima of ~555 580 nm [1], contains a carboxylic acid that reacts with primary amines via carbodiimide activation.

5(6)-Carboxy-2',7'-dichlorofluorescein serves as an excellent substrate for the MRP2 vesicular transport assay, offering superior detection and transport characteristics [1].

5(6)-Carboxynaphthofluorescein, a pH-dependent fluorophore, exhibits high sensitivity in alkaline pH settings, making it suitable for the development of fiber-optic pH sensors. Additionally, it serves as a fluorescent pH indicator with excitation emission wavelengths of 593 668 nm [1].

6-Carboxy-JF5252 is a fluorophore that can be used as probes, dyes, and tags for super-resolution imaging research [1].

6-Fluorescein phosphoramidite is a powerful fluorescent dye used for labeling oligonucleotides [1].

6-JOE, SE is a fluorescent probe that reacts with amines, making it ideal for labeling amino-modified oligonucleotides after synthesis [1].

6-TET Dipivaloate is utilized as a nucleic acid sequencing probe.

5-FAM-GpYLPQTV-NH2, a fluorescently labeled peptide, exhibits STAT3 inhibitory activity and is applicable in cancer research [1].

CypHer 5, a pH-sensitive cyanine derivative and environmental-sensitive fluorophore, exhibits pH-sensitive maximal absorption at 644 nm and emission at 664 nm. It has been utilized as a pH sensor for lysosomal compartments [1].

Reactive blue 5 is a common textile dye that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, facilitating the separation of residual dyes.

FAM amine, 6-isomer, is a fluorescein derivative with an amine group and an isomer of the fluorophore. It can modify biomolecules through enzymatic transamination and its fatty amine groups can react with electrophiles such as activated esters. Additionally, the amine can be conjugated to carbonyl compounds (aldehydes and ketones) by reductive amination.

N-[5-2-[Azo]phenyl]acetamide serves as a multifunctional dye used extensively across various fields. In the realm of biological experiments, this dye is instrumental for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its usage spans from fundamental scientific research to a broad spectrum of clinical diagnostics. Additionally, this compound finds applications in traditional industries like textile dyeing and emerging areas including functional textile processing, food pigments, and dye-sensitized solar cells.

Fluorescein-6-isothiocyanate is a useful organic compound for research related to life sciences. The catalog number is T64942 and the CAS number is 18861-78-4.

The isomeric 6-ROX, SE is an amine-reactive form of carboxy-X-rhodamine and is widely used for oligonucleotide labeling and automated DNA sequencing applications.

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient. 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.

Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 489 nm excitation wavelength. They are photostable alternative reag

5-ROX-alkyne, a rhodamine dye, labels DNA fragments and facilitates visualization in capillary electrophoresis genotyping and gel shift experiments.

Fluorescein-5-thiosemicarbazide is an amine-containing fluorescent probe used for labeling saccharides and protein carbonyl derivatives [1] [2].

TAMRA azide, 6-isomer, is an isomer of TAMRA, a xanthene dye that emits orange light.