4-methylumbelliferyl phosphate

4-Methylumbelliferyl phosphate (4-MUP) (4-MUP) is used as a fluorogenic substrate of alkaline phosphatases.

4-Methylumbelliferyl phosphate disodium (4-MUP), an anionic organophosphate, serves as a fluorogenic substrate for acid and alkaline phosphatases. Additionally, this compound acts as a simulant for nerve agents [1] [2] [3].

4-Methylumbelliferyl heptanoate (4-Methylumbelliferyl enanthate) is a lipase fluorescent substrate that detects cell-mediated cytotoxicity and cell proliferation.

4-Methylumbelliferyl 2-sulfamino-2-deoxy-α-D-Glucopyranoside (4-MU-α-GlcNS) is a fluorogenic substrate of heparin sulphamidase. Heparin sulphamidase cleaves 4-MU-α-GlcNS to yield 4-MU-α-GlcNH2, which is then cleaved by α-glucosaminidase to release the fluorescent product 4-MU, which displays an emission maxima of 445-454 nm. The excitation maxima for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. 4-MU-α-GlcNS has been used to quantify heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders.

4-Methylumbelliferyl β-cellotrioside, a cellulose-derived fluorescent chromogenic substrate [1], is specifically hydrolyzed by β-glycosidases.

4-Methylumbelliferyl palmitate serves as a potent fluorophore to assay acid lipase activity in human leukocytes, with its fluorescence significantly affected by acidity and solvent properties. In near-neutral aqueous solutions, it predominates in a neutral molecular form, emitting strong fluorescence at 445 nm, whereas in weakly alkaline solutions, it primarily exists in an anionic form, resulting in even stronger fluorescence at the same wavelength [1] [2].

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity. 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient. 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.

DiFMUP (6,8-Difluoro-4-methylumbelliferyl phosphate) inhibits protein tyrosine phosphatases (PTPs) and can be used to assay threonine phosphatase activity.

4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) is a fluorogenic substrat (λex=362 nm , λem=445 nm).

4-Methylumbelliferyl-α-L-fucopyranoside serves as a fluorogenic substrate for α-L-fucosidase, releasing 4-methylumbelliferone (4-ΜU) upon enzymatic cleavage by the enzyme. The fluorescence of 4-ΜU, with excitation/emission maxima at 350/440 nm, respectively, allows for the quantification of α-L-fucosidase activity.

4-Methylumbelliferyl-β-D-galactoside (MUG) is a fluorogenic substrate for β-galactosidase (β-gal).1Hydrolysis of MUG by β-gal releases the fluorescent moiety 4-MU, which displays a pH-dependent excitation maximum of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.2MUG has been used to detect β-gal activity in intact bacteria, yeast, and mammalian cells.1 1.Vidal-Aroca, F., Giannattasio, M., Brunelli, E., et al.One-step high-throughput assay for quantitative detection of β-galactosidase activity in intact Gram-negative bacteria, yeast, and mammalian cellsBiotechniques40(4)433-434(2006) 2.Zhi, H., Wang, J., Wang, S., et al.Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsuleJ. Spectrosc.1(1)147128(2013)

4-Methylumbelliferyl oleate is a fluorescent substrate for lipases (Ex/Em=320/450 nm), which can be cleaved by lipase and can be used to measure pancreatic inhibitory activity.